In vitro antioxidant and anticancer activity of leea. In brief, the plant extract solution 10 l was mixed with 100 l of folinciocalteus in a 96well. Production of silver nanoparticles synthesis of couroupita. Other nonconventional plant material we have studied was murta ugni molinae turcz. Production of silver nanoparticles synthesis of couroupita guianensis plant extract against human pathogen and evaluations of antioxidant properties sivakumar t, rathimeena t, shankar and ponmanickam p department of microbiology, ayya nadar janaki ammal college autonomous sivakasi626124, tamilnadu, india. The antioxidant activity of the aerial part extract of m. The obtained results showed that the extracting solvent significantly influenced the antioxidant property estimations of halimium halimifolium.
The table 2, 3, and 4 shows the antioxidant activities of the. Antioxidant activity of polyphenolic plant extracts. Different studies were carried out by comparing kalanchoe pinnata extract with antioxidant references such as gallic acid. Pdf methods for determining the antioxidant activity. Dec 11, 2019 the total free radical quenching dpph assay revealed the antioxidant activity of ic 50 9. Grape seed extract 95% procyanidolic oligomers or pine bark extract. Antioxidant activity of the leaf extract was assessed by dpph, cuprac and pfrap assays using lascorbic acid as standard.
The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Although the entire plant is known to have uses for human applications, it is the extracts of. Quality of an extract is inf luenced by sev eral factors such as, plant parts used as starting material, solve nt used for extraction, e x traction pr ocedure, and plant material. Estimation of phytochemical content and antioxidant activity. The total crude plant extracts were collected and evaporated to dryness by an oven. The flavonoid contents of ethanol extract had the higher amount of flavonoid contents than aqueous extract. Jul 19, 2010 abts radical cation was produced in the stable form using potassium persulphate. Biogas production efficiency was not correlated with the antioxidant activity of the crude extracts. Figure 1 shows the amount of each extract required for 50% inhibition of dpph activity ic 50.
Evaluation of antioxidant activity of medicinal plant 885 figure 2. After getting the stable absorbance, the antioxidant plant extract is added to the reaction medium and the antioxidant power was measured by studying decolorization. The superoxide anion scavenging activity of plant extracts was determined by the wst 24iodophenyl34nitrophenyl52,4disulphophenyl2htetrazolium, monosodium salt. Allium fistulosum bulb extract allium fistulosum, also known as the green onion or welsh onion, is a species of perennial plant that is very similar in taste and odor to the related common onion. Antioxidant activity and cytotoxicity of the leaf and bark extracts of. Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. Medicinal plants derived natural antioxidants, which are in the form of raw extracts andor chemical constituents, are very efficient to block the process of oxidation by neutralizing free radicals 3. Our aim was to evaluate total phenol and flavonoid contents, antioxidant capacity, free radical scavenging activity and potential antihypertensive effect of aqueous extract obtained from thymus serpyllum l.
Comparative study of antioxidant properties and total. Evidencebased complementary and alternative medicine hindawi. A comparative study on the antioxidant activity of methanolic. Screening of medicinal plant extracts for antioxidant activity.
The antioxidant capacity of the extract was expressed as mmol. Total phenolic, total flavonoid, tannin content, and. Aqueous extracts and formulations of white tea, witch hazel and rose were subjected to assays to measure anticollagenase. Antioxidant activity of plant extracts containing phenolic. Many plants and plant parts act as a good source of antioxidants.
It loses this absorption when reduced by an antioxidant or a free radical species. Antibacterial, antioxidant activity of ethanolic plant. The low incidence of cardiovascular disease in mediterranean countries leads to an increased interest of the scientific community for the mediterranean diet. These compounds are widespread in the plant kingdom as part of our daily diet and are attractive as natural antioxidants. The antioxidant activity of two extracts was tested by dpph 1,1diphenyl1. To improve the extraction efficiency of antioxidant components from plant materials, several green nonconventional methods have been. The scavenging effects of both plant extracts and the standard on the dpph radical were expressed as half maximal inhibitory concentration ic 50 values. Pdf different extracts from five medicinal plants in terms of phenolic compounds and antioxidant properties were studied. Antioxidant and antihypertensive activity of extract from. The extracts were analyzed for their total phenolic content tpc, total flavonoid content tfc, and antioxidant activity aa. The highest yield of solid residue was obtained using water or methanol as extraction solvents.
One gram of dry plant material was homogenized with 20 ml of solvent solution acetoneultrapure waterglacial acetic acid, 70. Standard curve was prepared using different concentrations 0. Assessment of antioxidant activity of plant extracts by. Antibacterial and antioxidant activity of plant latex. The yield of extract obtained from 10 g of dry plant material was measured for each extract table 1. This document discusses published information relevant to rosemary, rosemary. Extract, free radical, antioxidant activity, medicinal plant. Google scholar katalinic v, milos m, kulisic t, jukic m. Screening of in vitro antioxidant activity of methanolic. Preparation of plant extracts for antioxidant property analysis and total polyphenol content. Inhibition of microsomal peroxidation data represents, mean sem, for n3 the reducing power of different commercial extracts was performed and showed. The aim of this study was to assess the efficacies of three plant extracts for their potential antioxidant and antiinflammatory activity in primary human skin fibroblasts. Therefore, plant oils are widely used as a substrate for testing antioxidant activity.
Comparison of dpph and abts assays for determining. Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. Natural antioxidants, plant extracts, antioxidant activity, phenolic compounds. Antioxidant activity of some plant extracts of the family.
Evaluation of antioxidant capacity of plant extracts by. The many number of medicinal plants are used in the cellular and metabolic. Extract concentration that provide 50% inhibition ic 50 and expressed in g extract ml was calculated from the graph plotted inhibition percentages against tested samples extracts. Total phenols were estimated by folinciocalteus method and flavonoids by. Au515 a0a1 a0ka1k where au515 is the antiradical activity of the extract, a0 the absorbance of the sample at the beginning of the reaction 0 min, a1 the absorbance of the sample after incubation times 20120 sec of the reaction. Ae517 is the absorbance of the plant extract after 1 hour incubation. This is act by easing the level of endogenous defenses by up regulating the expression. Aug 19, 2015 javanmardi j, stushnoff c, locke e, vivanco jm. The plate was microscopically examined for confluent monolayer of cells, turbidity and toxicity. Abts radical cation was produced in the stable form using potassium persulphate. Plants having phenolic contents are reported to possess antioxidant properties. The present study evaluated the antioxidant effects of methanolic seed extract of a.
Antioxidant proprieties of methanolic and ethanolic extracts. The total free radical quenching dpph assay revealed the antioxidant activity of ic 50 9. On the other hand, the profile and quantity of phenolic compounds extracted from plant matrices changes depending on the species, cultivar, climate, and other. Among the important biological properties exhibited by plant polyphenols, their antioxidant activity has raised a great interest. Rosemary extract, 82nd jecfachemical and technical. In vitro antioxidant activity, total phenolic and flavonoid. Antioxidant activity and total phenolic content of iranian ocimum accessions. Decalepis hamiltonil is a plant from asclapiadaceae family. Numerous reports have identified therapeutic roles for plants and their extracts and constituents. Furthermore, to study the antioxidant activity of our plant extracts, the ability to scavenge the stable free radical dpph and the cation abts.
Plants have phenolic compounds with antioxidant activity. Moreover, the method could evaluate the antioxidant activity of rosemary extract in foods, and consequently its functional. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. May 29, 2018 antioxidants are mostly found in plant foods. The content of total phenolics in the extracts was determined spectrometrically according to the folin.
Antioxidant and potential antiinflammatory activity of. Antioxidant, anticancer activity and phytochemical analysis. Also specific for the lungs, diabetes, varicose veins, and protection against heart disease. The ethanol is a recommended solvent for extracting antioxidants from this plant. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase. The plant extract was tested by various model systems like dpph. Oct 02, 2010 2 ml extract was boiled with 2 ml of 1% hydrochloric acid hcl.
The antioxidant activity of the plant extract was then characterized using the dpph. Antioxidant properties of extracts from selected plant materials. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Antioxidant activity of plant extracts and their effect on. Numerous methods for the analysis of in vitro and in vivo antioxidant activity have been developed, but only a few fast and reliable methods for a large number of plant extract samples exist 3234. The antioxidant activity of murta leaves, according to phenols concentration in several extracts is shown in figure 1. Lastly, sugar maple extract has antioxidant properties that can neutralize free radicals, thereby protecting the skin from oxidative damage. The antioxidant activity of grewia carpinifolia extract may be due to the high level of flavonoids and.
The root extract of this plant exhibited a strong antioxidant capacity in the dpph assay ic 50. Antioxidant activity of aqueous, chloroform and methanol extract of the aerial parts of plant oftricholepis glaberrima dc were evaluated for the antioxidant activity by the ftc and tba methods. The scavenging activity of the plant extract through the annihilation of the dpph radicals was investigated. These compounds are distributed in tissues and cells of plants and their abundance depends on the species, the part of the plant used, maturity stage, light hours, among others. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Hence, the increase in methane fermentation efficiency in the. Antioxidant and in vitro cytotoxic activity of extracts of. Evaluation of the in vitro and in vivo antioxidant. Using the 1 mg ml concentration herbal extract, nine. The fresh working suspension was filtered through 0. They are natural molecules that help neutralize harmful free radicals in our bodies. Estimation of phytochemical content and antioxidant.
Natural antioxidants in foods and medicinal plants. Antioxidant activity, total phenolic and total flavonoid. Phytochemicals, antioxidant and antimicrobial potentials. Antioxidant properties of plant extracts edelweiss publications. Antioxidant, anticancer activity and phytochemical. Antioxidant activity of polyphenolic plant extracts mdpi. A number of studies have shown that plant polyphenols can be used as antioxidants against different oxidative stressinduced diseases 2,3.
Free radical scavenging by the superoxyde dismutase sod assay. Antioxidant potential of water and methanol 557 dpph is a stable free radical with an absorption band at 515 nm. Extraction, isolation and characterization of bioactive. The antioxidant activity of the latex extract was estimated using a slight modification of the dpph radical scavenging protocol reported by chen et al. The present study was designed to investigate the antioxidant properties and phenolic contents total phenols, flavonoids, flavonols and proanthrocyanidins of methanolic extracts from morus alba locally named as tut and. Samples of tragopogon porrifolius plant grown wild in palestine were extracted with different solvents. Plants 2017, 6, 42 2 of 23 of the lipid oxidation process, details of plants known to be antioxidant and antimicrobial sources, phenolic compounds, antioxidants from vegetables and fruits, cancer prevention, extraction techniques. The antioxidant activity of ethanolic extract of ca and cp was measured by the ability to scavenge dpph free radicals comparing with vitamin c. In these assays, ethanol and methanol were the best solvents and dpph inhibition percentages were very high for. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. The result showed that antioxidant activity of the extract was higher ascorbic acid with correspongding contents were 0.
Anticancer activity, antioxidant activity, and phenolic. The present study aims to evaluate the antioxidant and phytochemical potential of flowers of delonix regia. It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds cook and samman, 1996. Aqueous extracts of 30 plants were investigated for their antioxidant properties using dpph and abts radical scavenging capacity assay, oxygen radical absorbance capacity orac assay, superoxide dismutase sod assay, and ferric reducing antioxidant potential frap assay. Phytochemicals, antioxidant and antimicrobial potentials and. The free radical scavenging action of methanol extracts of plant are in the order as desmodium gangeticum amaranthus caudatus solanum nigrum. Total phenolic content was also determined by the folin. Evaluation for antioxidant capacity of ethanol extract of b. Evaluation of antioxidant activity of medicinal plant extracts. Free radicals are potentially harmful substances that result from. Antioxidant activities were evaluated in terms of total phenolics content, total antioxidant activity, and reducing power. Therefore to have the scientific validation on antioxidant properties, the leaves and. The highest content in phenols, flavonoids and tannins was shown in flowers extract 350 5 mg gaeg, 34 1 mg qeg and 221.
Antioxidant properties of several medicinal plants growing. The higher the ic 50, the lower is the antioxidant activity of the examined sample. Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer. Medicinal plants used in the traditional medicine are wellknown significant sources of natural antioxidants. A detailed study was performed on the antioxidant activity of the methanol extract of whole. Plant extracts as antioxidant additives for food industry.
Antioxidant proprieties of methanolic and ethanolic. Green tea extract 8090% total polyphenols 150300 mg. Antioxidants play an important role to protect damage caused by oxidative stress os. Screening of in vitro antioxidant activity of methanolic leaf. Determination of total phenolic content total phenolic content was determined through a folinciocalteu colorimetric method, modified from mayur et al. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata l. Dpph free radical scavenging activity of the extracts of the. Dpph method is widely used to determine antiradical antioxidant activity of purified phenolic compounds. Comparatively, methanolic extract showed higher activity than ethanolic activity. The concentration of extracts in lard varied from 0.
Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural systems, plant biotechnology and. Evaluation of antioxidant activity of medicinal plant. Dpph free radical scavenging activity of the extracts of. Issn total antioxidant capacity tac of fresh leaves of. To obtain the extracts the methodology previously described by michiels et al. Methanolic extract showed higher antioxidant activity than the chloroform and aqueous extract.
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